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Click–Click–Click: Single to Triple Modification of DNA
Author(s) -
Gramlich Philipp M. E.,
Warncke Simon,
Gierlich Johannes,
Carell Thomas
Publication year - 2008
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.200705664
Subject(s) - phosphoramidite , click chemistry , surface modification , combinatorial chemistry , dna , modular design , computer science , chemistry , oligonucleotide , programming language , biochemistry
One, two, or three : An efficient, modular, and robust protocol has been developed for the multiple functionalization of DNA. It is based on the click reaction of azides with the alkyne substituents on an oligodeoxyribonucleotide (ODN), which was prepared by the standard phosphoramidite method (see scheme). ODNs can thus be labeled with two sensitive molecules, and even triple modification is possible.