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An Efficient Fmoc‐SPPS Approach for the Generation of Thioester Peptide Precursors for Use in Native Chemical Ligation
Author(s) -
BlancoCanosa Juan B.,
Dawson Philip E.
Publication year - 2008
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.200705471
Subject(s) - native chemical ligation , thioester , chemistry , chemical ligation , peptide , intramolecular force , ligation , side chain , combinatorial chemistry , amino acid , peptide synthesis , chemical synthesis , stereochemistry , biochemistry , organic chemistry , microbiology and biotechnology , biology , in vitro , enzyme , polymer
Intramolecular activation of C‐terminal peptides: Mildly activated N ‐acylurea peptides are readily formed on the solid support following chain assembly, avoiding over‐activation of the C‐terminus. The utility of these peptides is demonstrated by the native chemical ligation of unprotected peptides (see scheme; R=amino acid side chain).