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A Near‐Infrared‐Fluorescence‐Quenched Gold‐Nanoparticle Imaging Probe for In Vivo Drug Screening and Protease Activity Determination
Author(s) -
Lee Seulki,
Cha EuiJoon,
Park Kyeongsoon,
Lee SeungYoung,
Hong JinKi,
Sun InCheol,
Kim Sang Yoon,
Choi Kuiwon,
Kwon Ick Chan,
Kim Kwangmeyung,
Ahn CheolHee
Publication year - 2008
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.200705240
Subject(s) - in vivo , matrix metalloproteinase , conjugated system , in vitro , fluorescence , colloidal gold , protease , chemistry , nanoparticle , contrast (vision) , biophysics , nanotechnology , combinatorial chemistry , materials science , biochemistry , enzyme , biology , optics , organic chemistry , physics , polymer , microbiology and biotechnology
Nanodiagnosis: A matrix‐metalloproteinase (MMP) sensitive gold‐nanoparticle (AuNP) imaging probe quenches conjugated near‐infrared (NIR) dyes with high efficiency and is specifically activated by the target MMPs (see picture, left). With this system, nanomolar amounts of protease can be detected—both in vitro and in vivo. Experiments disclose an apparent positive contrast in MMPs‐positive tumor‐bearing mice (right).