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Single‐Cell High‐Throughput Screening To Identify Enantioselective Hydrolytic Enzymes
Author(s) -
Becker Stefan,
Höbenreich Horst,
Vogel Andreas,
Knorr Janina,
Wilhelm Susanne,
Rosenau Frank,
Jaeger KarlErich,
Reetz Manfred T.,
Kolmar Harald
Publication year - 2008
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.200705236
Subject(s) - enantioselective synthesis , esterase , throughput , high throughput screening , computer science , peroxidase , enantiomer , chemistry , combinatorial chemistry , computational biology , enzyme , identification (biology) , hydrolysis , information retrieval , biochemistry , stereochemistry , biology , catalysis , botany , telecommunications , wireless
Getting a look in : A high‐throughput screening method has been developed for the identification and isolation of enantioselective hydrolases displayed on cell surfaces (see scheme; E: Esterase, P: Peroxidase). Enantiomeric substrates labeled with two different fluorescent dyes allow real‐time analysis of enantioselectivity by determination of the ratio of green and red single‐cell fluorescence.