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Synthesis and DNA‐Polymerase Incorporation of Colored 4‐Selenothymidine Triphosphate for Polymerase Recognition and DNA Visualization
Author(s) -
CatonWilliams Julianne,
Huang Zhen
Publication year - 2008
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.200705213
Subject(s) - dna polymerase , dna , polymerase , chemistry , biochemistry , microbiology and biotechnology , biology
Highlighting changes in yellow : The replacement of a single oxygen atom in thymidine triphosphate with a selenium atom gave yellow 4‐selenothymidine 5′‐triphosphate ( Se TTP; see solutions of colorless natural TTP (left) and Se TTP (right)). Se TTP is recognized by DNA polymerase. Its incorporation into DNA (see scheme) yields colored DNA and occurs with the same level of efficiency as the incorporation of natural TTP.