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Clockwork PCR Including Sample Preparation
Author(s) -
Pipper Juergen,
Zhang Yi,
Neuzil Pavel,
Hsieh TsengMing
Publication year - 2008
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.200705016
Subject(s) - clockwork , superparamagnetism , sample (material) , chemistry , computer science , chromatography , nanotechnology , computational biology , microbiology and biotechnology , physics , materials science , biology , magnetization , quantum mechanics , astronomy , magnetic field
For whom the bell tolls : Surface‐functionalized superparamagnetic particles emulsified in mineral oil turn a free‐standing droplet into a flexible virtual laboratory with (sub)microliter volumes. By using magnetic forces, rare acute monocytic leukaemia cells are extracted from blood, preconcentrated, purified, lysed, and subjected to a real‐time PCR in minutes. The PCR works like a clockwork by rotating the droplet over different temperature zones.