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X‐Ray Structure and Designed Evolution of an Artificial Transfer Hydrogenase
Author(s) -
Creus Marc,
Pordea Anca,
Rossel Thibaud,
Sardo Alessia,
Letondor Christophe,
Ivanova Anita,
LeTrong Isolde,
Stenkamp Ronald E.,
Ward Thomas R.
Publication year - 2008
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.200704865
Subject(s) - hydrogenase , ruthenium , selectivity , coordination sphere , chemistry , artificial photosynthesis , directed molecular evolution , catalysis , biological system , combinatorial chemistry , crystallography , biology , crystal structure , directed evolution , gene , biochemistry , photocatalysis , mutant
A structure is worth a thousand words : Guided by the X‐ray structure of an S ‐selective artificial transfer hydrogenase, designed evolution was used to optimize the selectivity of hybrid catalysts. Fine‐tuning of the second coordination sphere of the ruthenium center (see picture, orange sphere) by introduction of two point mutations allowed the identification of selective artificial transfer hydrogenases for the reduction of dialkyl ketones.