Premium
Self‐Sufficient Baeyer–Villiger Monooxygenases: Effective Coenzyme Regeneration for Biooxygenation by Fusion Engineering
Author(s) -
Torres Pazmiño Daniel E.,
Snajdrova Radka,
Baas BertJan,
Ghobrial Michael,
Mihovilovic Marko D.,
Fraaije Marco W.
Publication year - 2008
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.200704630
Subject(s) - monooxygenase , cofactor , nicotinamide adenine dinucleotide phosphate , biochemistry , biocatalysis , chemistry , enzyme , dehydrogenase , nicotinamide adenine dinucleotide , nad+ kinase , cytochrome p450 , catalysis , oxidase test , ionic liquid
Two‐in‐one biocatalysts were engineered by the covalent fusion of NADPH‐dependent Baeyer–Villiger monooxygenases to a phosphite dehydrogenase for coenzyme regeneration (see scheme). Not only the purified fusion proteins, but also whole cells and crude cell extracts containing the enzyme conjugates, could be used to catalyze biotransformations with high efficiency. NADP + =nicotinamide adenine dinucleotide phosphate.