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Double Electron–Electron Resonance (DEER): A Convenient Method To Probe DNA Conformational Changes
Author(s) -
Sicoli Giuseppe,
Mathis Gérald,
Delalande Olivier,
Boulard Yves,
Gasparutto Didier,
Gambarelli Serge
Publication year - 2008
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.200704133
Subject(s) - dna , electron , chemistry , z dna , electron paramagnetic resonance , biophysics , crystallography , nuclear magnetic resonance , chemical physics , physics , biochemistry , biology , quantum mechanics
Oh DEER! The DEER technique is capable of detecting even slight conformational changes in site‐directed spin‐labeled DNA structures, which are extremely difficult to measure by more classical methods. The B–A transition (see picture) in DNA duplexes is induced by trifluoroethanol. DEER can be applied to the study of modifications of DNA induced by various agents.