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Addition of an α‐Hydroxy Acid to the Genetic Code of Bacteria
Author(s) -
Guo Jiantao,
Wang Jiangyun,
Anderson J. Christopher,
Schultz Peter G.
Publication year - 2008
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.200704074
Subject(s) - genetic code , nonsense mutation , chemistry , nonsense , bacteria , hydrolysis , biochemistry , escherichia coli , amino acid , dna , biology , genetics , stereochemistry , mutation , gene , missense mutation
Playing tag : The α‐hydroxy acid p ‐hydroxy‐ L ‐phenyllactic acid has been genetically incorporated into proteins in E. coli in response to an amber nonsense codon. The site‐specific introduction of backbone ester mutations was used for the site‐specific hydrolysis of an affinity tag, as well as for determination of the energetic contributions of backbone hydrogen bonds to protein stability.