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DNA Minicircles with Gaps for Versatile Functionalization
Author(s) -
Rasched Goran,
Ackermann Damian,
Schmidt Thorsten L.,
Broekmann Peter,
Heckel Alexander,
Famulok Michael
Publication year - 2008
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.200704004
Subject(s) - minicircle , oligonucleotide , dna , sequence (biology) , computational biology , dna sequencing , biology , nanotechnology , materials science , genetics
Doing the rounds : A highly straightforward approach has been developed that provides access to DNA minicircles containing a 21‐mer single‐stranded gap region of defined sequence (see picture). The single‐stranded domain within a small circular DNA nanoobject provides the exciting opportunity to further functionalize a minicircle at defined positions in almost unlimited fashion, simply by hybridization with a synthetic 21‐mer oligonucleotide.