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Cellular Uptake Quantification of Metalated Peptide and Peptide Nucleic Acid Bioconjugates by Atomic Absorption Spectroscopy
Author(s) -
Kirin Srecko I.,
Ott Ingo,
Gust Ronald,
Mier Walter,
Weyhermüller Thomas,
MetzlerNolte Nils
Publication year - 2008
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.200703994
Subject(s) - atomic absorption spectroscopy , chemistry , peptide nucleic acid , nucleic acid , peptide , spectroscopy , metal , absorption (acoustics) , absorption spectroscopy , cobalt , conjugate , inorganic chemistry , biochemistry , materials science , organic chemistry , physics , quantum mechanics , composite material , mathematical analysis , mathematics
The metal does the trick! Atomic absorption spectroscopy (AAS) of cobalt atoms is used as an accurate method to determine the cellular uptake and nuclear localization of metal bioconjugates. Surprisingly, the PNA conjugates show highest uptake efficiency, and an accumulation 150 % higher than in the culture medium is achieved.