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Probing Inducible Nitric Oxide Synthase with a Pterin–Ruthenium(II) Sensitizer Wire
Author(s) -
Glazer Edith C.,
Nguyen Yen Hoang Le,
Gray Harry B.,
Goodin David B.
Publication year - 2008
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.200703743
Subject(s) - pterin , tetrahydrobiopterin , chemistry , ruthenium , heme , nitric oxide synthase , cofactor , photochemistry , nitric oxide , stereochemistry , biochemistry , catalysis , enzyme , organic chemistry
Binding by the light : A photoactive ruthenium(II)–pterin wire designed to target the tetrahydrobiopterin (H 4 B) binding site of inducible nitric oxide synthase (iNOS heme ) binds to iNOS heme in a competitive fashion with the natural cofactor and other small molecules; thus it can be used to screen potential inhibitors. Rapid charge injection to the heme occurs upon 450‐nm excitation of an iNOS heme /wire conjugate.