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Diazirine‐Based DNA Photo‐Cross‐Linking Probes for the Study of Protein–DNA Interactions
Author(s) -
Shigdel Uddhav Kumar,
Zhang Junliang,
He Chuan
Publication year - 2007
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.200703625
Subject(s) - diazirine , dna , steric effects , chemistry , groove (engineering) , minor groove , biophysics , biochemistry , stereochemistry , biology , engineering , mechanical engineering
Small modification, large effect : Diazirine can be installed quickly and simply in the major or minor groove of DNA for efficient photo‐cross‐linking to proteins (see picture). This method should be suitable for mapping out protein–DNA interactions, in particular those that may be sensitive to steric hindrance.