Premium
A Tunable FRET Circuit for Engineering Fluorescent Biosensors
Author(s) -
Allen Michael D.,
Zhang Jin
Publication year - 2008
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.200703493
Subject(s) - förster resonance energy transfer , biosensor , fluorophore , fluorescence , acceptor , arbiter , tuner , nanotechnology , computer science , chemistry , materials science , physics , telecommunications , computer hardware , condensed matter physics , radio frequency , quantum mechanics
Donor, acceptor, and tuner : FRET‐based ratiometric live‐cell imaging captures emissions of two fluorophores within the constraints of a FRET pair. A method for engineering FRET‐circuit‐based biosensors has been developed by introducing an intermediate fluorophore, which facilitates energy transfer and allows tuning of the circuit efficiency. A protein kinase A biosensor was thus generated with a larger dynamic range than its precursors.