A Tunable FRET Circuit for Engineering Fluorescent Biosensors | Zendy

Allen Michael D. | Zendy; Zhang Jin | Zendy

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A Tunable FRET Circuit for Engineering Fluorescent Biosensors

Author(s) -

Allen Michael D.,

Zhang Jin

Publication year - 2008

Publication title -

angewandte chemie international edition

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 5.831

H-Index - 550

eISSN - 1521-3773

pISSN - 1433-7851

DOI - 10.1002/anie.200703493

Subject(s) - förster resonance energy transfer , biosensor , fluorophore , fluorescence , acceptor , arbiter , tuner , nanotechnology , computer science , chemistry , materials science , physics , telecommunications , computer hardware , condensed matter physics , radio frequency , quantum mechanics

Donor, acceptor, and tuner : FRET‐based ratiometric live‐cell imaging captures emissions of two fluorophores within the constraints of a FRET pair. A method for engineering FRET‐circuit‐based biosensors has been developed by introducing an intermediate fluorophore, which facilitates energy transfer and allows tuning of the circuit efficiency. A protein kinase A biosensor was thus generated with a larger dynamic range than its precursors.

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