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A Genetically Encoded Bidentate, Metal‐Binding Amino Acid
Author(s) -
Xie Jianming,
Liu Wenshe,
Schultz Peter G.
Publication year - 2007
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.200703397
Subject(s) - denticity , amino acid , nonsense , transfer rna , yield (engineering) , computational biology , chemistry , biochemistry , gene , genetics , biology , metal , organic chemistry , materials science , rna , metallurgy
A two‐ring binder : To facilitate the design of metalloproteins, the bidentate, metal‐binding amino acid bipyridylalanine (BpyAla) was genetically encoded in E. coli in response to the amber nonsense codon with high fidelity and yield. The incorporation of BpyAla requires a BpyAla‐specific aminoacyl‐tRNA synthetase, which was evolved in a stepwise fashion. The structural basis of selective recognition of BpyAla by this synthetase was also determined.