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Interaction of Malachite Green with Guanine‐Rich Single‐Stranded DNA: Preferential Binding to a G‐Quadruplex
Author(s) -
Bhasikuttan Achikanath C.,
Mohanty Jyotirmayee,
Pal Haridas
Publication year - 2007
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.200703251
Subject(s) - malachite green , g quadruplex , guanine , oligomer , dna , chemistry , ionic strength , yield (engineering) , biophysics , fluorescence , sequence (biology) , crystallography , biochemistry , materials science , polymer chemistry , biology , organic chemistry , aqueous solution , gene , adsorption , metallurgy , nucleotide , physics , quantum mechanics
Bound to be better : The formation of a strong complex between the chromophoric dye malachite green (MG, in green) and the G‐quadruplex structure (represented in black) of the guanine‐rich single‐strand oligomer sequence d(G 2 T) 13 G results in a 100‐fold enhancement of the fluorescence yield of MG. The existence of an intra‐ or interstrand G‐quadruplex structure depends on the oligomer concentration and the ionic strength of the solution.