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Ultrasensitive Label‐Free Aptamer‐Based Electronic Detection
Author(s) -
Xiang Yun,
Xie Mingyi,
Bash Ralph,
Chen Julian J. L.,
Wang Joseph
Publication year - 2007
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.200703242
Subject(s) - aptamer , chemistry , guanine , nanotechnology , thrombin , combinatorial chemistry , computational biology , microbiology and biotechnology , biochemistry , nucleotide , materials science , biology , platelet , gene , immunology
The highs and lows of proteins : A dramatically amplified aptamer‐based bioelectronic assay has been developed that allows the ultrasensitive measurement of thrombin down to the femtomolar level. This label‐free electronic detection of thrombin takes advantage of the intrinsic electroactivity of a second aptamer with guanine bases, and the enormous amplification feature of the polymerase chain reaction (PCR, see picture).