Premium
Rapid Detection of S ‐Adenosyl Homocysteine Using Self‐Assembled Optical Diffraction Gratings
Author(s) -
Acharya Ghanashyam,
Chang ChunLi,
Holland David P.,
Thompson David H.,
Savran Cagri A.
Publication year - 2008
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.200703222
Subject(s) - diffraction , conjugated system , detection limit , materials science , optics , nanotechnology , chemistry , optoelectronics , physics , chromatography , polymer , organic chemistry
I saw the SAH : Clinically relevant concentrations of S ‐adenosyl homocysteine (SAH) can be rapidly detected using self‐assembled diffraction gratings. SAH bound to antibody‐conjugated (Ab) microbeads (see picture) and then incubated with a gold chip functionalized with SAH‐specific aptamer in alternating line patterns produces gratings that are detected by optical diffraction intensity measurements (detection limit: 64 pmol L −1 ).