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Fluorous‐Based Small‐Molecule Microarrays for the Discovery of Histone Deacetylase Inhibitors
Author(s) -
Vegas Arturo J.,
Bradner James E.,
Tang Weiping,
McPherson Olivia M.,
Greenberg Edward F.,
Koehler Angela N.,
Schreiber Stuart L.
Publication year - 2007
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.200703198
Subject(s) - histone deacetylase , small molecule , histone , dna microarray , computational biology , chemistry , microarray , surface plasmon resonance , enzyme , biochemistry , biology , nanotechnology , gene , gene expression , materials science , nanoparticle
Noncovalent immobilization is an attractive method for identifying inhibitors of histone deacetylases (HDACs). Fluorous‐based small‐molecule microarrays were validated as an effective method. Three enzymes and three assays (microarray, biochemical activity, and surface plasmon resonance) were used to identify inhibitors of HDACs and to compare them.