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A Submicrogram‐Scale Protocol for Biomolecule‐Based PET Imaging by Rapid 6π‐Azaelectrocyclization: Visualization of Sialic Acid Dependent Circulatory Residence of Glycoproteins
Author(s) -
Tanaka Katsunori,
Masuyama Tatsuro,
Hasegawa Koki,
Tahara Tsuyoshi,
Mizuma Hiroshi,
Wada Yasuhiro,
Watanabe Yasuyoshi,
Fukase Koichi
Publication year - 2007
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.200702989
Subject(s) - sialic acid , chemistry , orosomucoid , glycoprotein , monoclonal antibody , dota , biochemistry , computational biology , antibody , medicine , chelation , biology , immunology , organic chemistry
On target : Lysine residues in picomole samples of a peptide, proteins, and a monoclonal antibody were rapidly and selectively labeled by a dota‐based probe ( 1 , see scheme). Positron emission tomography imaging of [ 68 Ga]dota‐labeled orosomucoid and asialoorosomucoid for the first time visualized a difference in the clearance process of glycoproteins in the presence or absence of the sialic acid residue.