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Solid‐State Protein‐Structure Determination with Proton‐Detected Triple‐Resonance 3D Magic‐Angle‐Spinning NMR Spectroscopy
Author(s) -
Zhou Donghua H.,
Shea John J.,
Nieuwkoop Andrew J.,
Franks W. Trent,
Wylie Benjamin J.,
Mullen Charles,
Sandoz Dennis,
Rienstra Chad M.
Publication year - 2007
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.200702905
Subject(s) - magic angle spinning , microcrystalline , spinning , nuclear magnetic resonance spectroscopy , solid state nuclear magnetic resonance , spectroscopy , magic (telescope) , magic angle , chemistry , physics , analytical chemistry (journal) , materials science , crystallography , nuclear magnetic resonance , organic chemistry , quantum mechanics , polymer chemistry
Spinning a magical web : The combination of fast magic‐angle spinning, isotopic dilution, and high magnetic field yields particularly well‐resolved solid‐state 1 H NMR spectra, which are efficiently utilized to solve protein structure. New techniques are demonstrated, requiring only three days of data collection, to assign the proton signals and solve a high‐resolution structure of microcrystalline GB1.