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Engineered Alkane‐Hydroxylating Cytochrome P450 BM3 Exhibiting Nativelike Catalytic Properties
Author(s) -
Fasan Rudi,
Chen Mike M.,
Crook Nathan C.,
Arnold Frances H.
Publication year - 2007
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.200702616
Subject(s) - propane , hydroxylation , domain (mathematical analysis) , catalysis , alkane , cytochrome p450 , chemistry , cytochrome , organic chemistry , enzyme , computer science , stereochemistry , combinatorial chemistry , mathematics , mathematical analysis
Divide, evolve, and conquer : A domain‐based strategy (see scheme) was used to engineer high catalytic and coupling efficiency for propane hydroxylation in a multidomain cytochrome P450 enzyme. The engineered enzymes exhibit high total activities in whole‐cell bioconversions of propane to propanol under mild conditions, using air as oxidant.