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A Biosynthetic Route to Dehydroalanine‐Containing Proteins
Author(s) -
Wang Jiangyun,
Schiller Stefan M.,
Schultz Peter G.
Publication year - 2007
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.200702305
Subject(s) - dehydroalanine , nonsense , chemistry , biochemistry , amino acid , stop codon , green fluorescent protein , fluorescence , gene , physics , quantum mechanics
It's not natural : The nonnatural amino acid phenylselenocysteine has been genetically incorporated into proteins in E. coli in response to the amber nonsense codon TAG. Oxidative elimination followed by Michael addition led to incorporation of ( S )‐hexadecyl‐ and ( S )‐mannosylcysteine into green fluorescent protein. This approach provides a useful method to modify protein side chains and backbone structures.