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Engineering a Selective Small‐Molecule Substrate Binding Site into a Deoxyribozyme
Author(s) -
Höbartner Claudia,
Silverman Scott K.
Publication year - 2007
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.200702217
Subject(s) - deoxyribozyme , substrate (aquarium) , small molecule , nucleic acid , chemistry , dna ligase , rna , molecule , binding site , combinatorial chemistry , biochemistry , nanotechnology , stereochemistry , enzyme , computational biology , biology , dna , materials science , organic chemistry , gene , ecology
A small goal: An RNA ligase deoxyribozyme is engineered to accept a small‐molecule NTP substrate in a multiple‐turnover fashion. Selective binding is enforced by hydrogen bonding, and structural preorganization within the NTP itself is important for its efficient utilization as a substrate. This study points the way toward a broader use of small‐molecule substrates with nucleic acid enzymes.