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A Microarray Strategy for Mapping the Substrate Specificity of Protein Tyrosine Phosphatase
Author(s) -
Köhn Maja,
GutierrezRodriguez Marta,
Jonkheijm Pascal,
Wetzel Stefan,
Wacker Ron,
Schroeder Hendrik,
Prinz Heino,
Niemeyer Christof M.,
Breinbauer Rolf,
Szedlacsek Stefan E.,
Waldmann Herbert
Publication year - 2007
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.200701601
Subject(s) - protein tyrosine phosphatase , docking (animal) , peptide , biochemistry , tyrosine , phosphatase , substrate specificity , dna microarray , chemistry , protein microarray , computational biology , binding site , substrate (aquarium) , enzyme , biology , gene , medicine , gene expression , ecology , nursing
Which substrate will it be? Phosphotyrosine peptide microarrays have allowed the substrate specificity to be mapped for two prototypical protein‐tyrosine phosphatases (PTPs): PTP1B and PTPμ. The knowledge gained was used for molecular docking studies (see picture: the docking of a peptide in the binding pocket of PTPμ) and the design of an inhibitor for PTPμ.