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Squaraine Rotaxanes: Superior Substitutes for Cy‐5 in Molecular Probes for Near‐Infrared Fluorescence Cell Imaging
Author(s) -
Johnson James R.,
Fu Na,
Arunkumar Easwaran,
Leevy W. Matthew,
Gammon Seth T.,
PiwnicaWorms David,
Smith Bradley D.
Publication year - 2007
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.200701491
Subject(s) - fluorophore , fluorescence , quenching (fluorescence) , fluorescence lifetime imaging microscopy , chemistry , photochemistry , bodipy , nanotechnology , biophysics , materials science , optics , physics , biology
It's hip to be square : Squaraine rotaxanes have very similar photophysical properties to the commonly used Cy‐5 fluorophore, but are substantially more photostable and resist self‐quenching upon aggregation. Molecular probes containing squaraine rotaxanes (see structure) are shown to be versatile, high‐performance NIR fluorescence stains for in vitro fluorescence imaging of cells (middle) and in vivo whole‐body imaging of living mice (right).