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Protein Kinase Assay on Peptide‐Conjugated Gold Nanoparticles by Using Secondary‐Ion Mass Spectrometric Imaging
Author(s) -
Kim YoungPil,
Oh Eunkeu,
Oh YoungHee,
Moon Dae Won,
Lee Tae Geol,
Kim HakSung
Publication year - 2007
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.200701418
Subject(s) - peptide , conjugated system , colloidal gold , chemistry , mass spectrometry , nanoparticle , protein kinase a , chromatography , kinase , biochemistry , materials science , nanotechnology , organic chemistry , polymer
Spot the mass of peptides : A label‐free protein kinase assay that uses secondary‐ion mass spectrometric imaging has been demonstrated with peptide‐conjugated gold nanoparticles (AuNPs). With detection of the mass change of peptide substrates in a kinase reaction, AuNP‐enhanced peptide signals enabled the assaying of both the protein kinase and its inhibition by chemical imaging of peptide substrates on a surface (see picture).