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Forty Years of In Vitro Evolution
Author(s) -
Joyce Gerald F.
Publication year - 2007
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.200701369
Subject(s) - darwinism , rna world hypothesis , chemical evolution , rna , evolutionary biology , systematic evolution of ligands by exponential enrichment , biology , computational biology , computer science , genetics , gene , ribozyme , stars , computer vision
It has been 40 years since Spiegelman and co‐workers demonstrated how RNA molecules can be evolved in the test tube. This result established Darwinian evolution as a chemical process and paved the way for the many directed evolution experiments that followed. Chemists can benefit from reflecting on Spiegelman's studies and the subsequent advances, which have taken the field to the brink of the generation of life itself in the laboratory. This Review summarizes the concepts and methods for the directed evolution of RNA molecules in vitro.