Premium
Positional Assembly of Enzymes in Polymersome Nanoreactors for Cascade Reactions
Author(s) -
Vriezema Dennis M.,
Garcia Paula M. L.,
Sancho Oltra Núria,
Hatzakis Nikos S.,
Kuiper Suzanne M.,
Nolte Roeland J. M.,
Rowan Alan E.,
van Hest Jan C. M.
Publication year - 2007
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.200701125
Subject(s) - nanoreactor , polymersome , horseradish peroxidase , glucose oxidase , chemistry , copolymer , abts , biocatalysis , lipase , combinatorial chemistry , candida antarctica , amphiphile , enzyme , chemical engineering , polymer chemistry , organic chemistry , catalysis , polymer , ionic liquid , antioxidant , dpph , engineering
In a good position : Nanoreactors can be constructed by the controlled positioning of glucose oxidase (GOX) and horseradish peroxidase (HRP) within the central water pool and block‐copolymer membrane of polymersomes. A one‐pot multistep reaction sequence is performed with the nanoreactor in combination with free Candida antarctica lipase B (CALB) in the bulk solution (see picture; ABTS: 2,2′‐azinobis(3‐ethylbenzothiazoline‐6‐sulfonic acid)).