Discrimination and Selective Enhancement of Signals in the MALDI Mass Spectrum of a Protein by Combining a Matrix‐Based Label for Lysine Residues with a Neutral Matrix | Zendy

Lascoux David | Zendy; Paramelle David | Zendy; Subra Gilles | Zendy; Heymann Michaël | Zendy; Geourjon Christophe | Zendy; Martinez Jean | Zendy; Forest Eric | Zendy

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Discrimination and Selective Enhancement of Signals in the MALDI Mass Spectrum of a Protein by Combining a Matrix‐Based Label for Lysine Residues with a Neutral Matrix

Author(s) -

Lascoux David,

Paramelle David,

Subra Gilles,

Heymann Michaël,

Geourjon Christophe,

Martinez Jean,

Forest Eric

Publication year - 2007

Publication title -

angewandte chemie international edition

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 5.831

H-Index - 550

eISSN - 1521-3773

pISSN - 1433-7851

DOI - 10.1002/anie.200700811

Subject(s) - lysine , reagent , chemistry , matrix (chemical analysis) , mass spectrum , mass spectrometry , chromatography , organic chemistry , amino acid , biochemistry

Discrimination with a difference : The N ‐hydroxysuccinimide ester of HCCA (α‐cyano‐4‐hydroxycinnamic acid) was used as a labeling reagent to increase the MALDI MS signal of weakly concentrated peptides of interest in a proteolytic mixture derived from cytochrome c relative to the signals of other peptides. The desired effect was only observed with the neutral MALDI matrix HCCE (the methyl ester of HCCA; see mass spectra).

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