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Discrimination and Selective Enhancement of Signals in the MALDI Mass Spectrum of a Protein by Combining a Matrix‐Based Label for Lysine Residues with a Neutral Matrix
Author(s) -
Lascoux David,
Paramelle David,
Subra Gilles,
Heymann Michaël,
Geourjon Christophe,
Martinez Jean,
Forest Eric
Publication year - 2007
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.200700811
Subject(s) - lysine , reagent , chemistry , matrix (chemical analysis) , mass spectrum , mass spectrometry , chromatography , organic chemistry , amino acid , biochemistry
Discrimination with a difference : The N ‐hydroxysuccinimide ester of HCCA (α‐cyano‐4‐hydroxycinnamic acid) was used as a labeling reagent to increase the MALDI MS signal of weakly concentrated peptides of interest in a proteolytic mixture derived from cytochrome c relative to the signals of other peptides. The desired effect was only observed with the neutral MALDI matrix HCCE (the methyl ester of HCCA; see mass spectra).