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Polymer‐Based Elemental Tags for Sensitive Bioassays
Author(s) -
Lou Xudong,
Zhang Guohua,
Herrera Isaac,
Kinach Robert,
Ornatsky Olga,
Baranov Vladimir,
Nitz Mark,
Winnik Mitchell A.
Publication year - 2007
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.200700796
Subject(s) - bioassay , polymer , environmental science , environmental chemistry , chemistry , biology , ecology , organic chemistry
It's elemental : A water‐soluble polymer bearing multiple metal‐chelating ligands has been used as a tag for bioassays with inductively coupled plasma mass spectrometry. The tag was covalently conjugated to antibodies, and the polymer–antibody constructs were loaded with lanthanide ions (Ln 3+ ) and used for the simultaneous assay of five orthogonally labeled antibodies against cell surface antigens that differ in abundance by more than two orders of magnitude.