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High‐Throughput Screening for Methionyl‐tRNA Synthetases That Enable Residue‐Specific Incorporation of Noncanonical Amino Acids into Recombinant Proteins in Bacterial Cells
Author(s) -
Yoo Tae Hyeon,
Tirrell David A.
Publication year - 2007
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.200700779
Subject(s) - recombinant dna , transfer rna , amino acid , methionine , biochemistry , residue (chemistry) , mutant , high throughput screening , computational biology , chemistry , biology , gene , rna
A change for the better : An efficient method for the identification of mutant methionyl‐tRNA synthetases (MetRS) has been developed that enables global incorporation of noncanonical amino acids into recombinant proteins. By using the method, an MetRS variant has been identified that enables near‐quantitative replacement of methionine by 6,6,6‐trifluoronorleucine (see scheme).