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Protein‐Phosphorylation‐Responsive Polymeric Nanoparticles for Imaging Protein Kinase Activities in Single Living Cells
Author(s) -
Kim JongHo,
Lee Seulki,
Park Kyeongsoon,
Nam Hae Yun,
Jang Soon Young,
Youn Inchan,
Kim Kwangmeyung,
Jeon Hyesung,
Park RangWoon,
Kim InSan,
Choi Kuiwon,
Kwon Ick Chan
Publication year - 2007
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.200700767
Subject(s) - protein kinase a , förster resonance energy transfer , protein phosphorylation , kinase , biochemistry , chemistry , cyclin dependent kinase 2 , map2k7 , protein kinase inhibitor , fluorophore , phosphorylation , microbiology and biotechnology , biology , fluorescence , physics , quantum mechanics
Take your PIC : Cell‐permeable and biocompatible polyion‐induced complex (PIC) nanoparticles were developed consisting of a negatively charged polymer along with a positively charged polymer conjugated to a near‐infrared fluorophore and a protein‐kinase‐specific peptide. Owing to profound changes in fluorescence, the PIC nanoparticle is an attractive probe for protein kinase A (PKA) in single living cells. ATP=adenosine triphosphate, ADP=adenosine diphosphate.