Premium
Regioselective Cysteine Bioconjugation by Appending a Labeled Cystein Tag to a Protein by Using Protein Splicing in trans
Author(s) -
Kurpiers Thomas,
Mootz Henning D.
Publication year - 2007
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.200700719
Subject(s) - bioconjugation , regioselectivity , cysteine , chemistry , chemical biology , trans splicing , protein–protein interaction , combinatorial chemistry , rna splicing , biochemistry , enzyme , gene , rna , catalysis
Orthogonal cysteines : The chemical modification of cysteine residues is one of the most important methods for the chemoselective bioconjugation of proteins; however, it lacks regioselectivity. By assembling the protein from two pieces using protein trans ‐splicing, regioselectivity can be achieved. This reaction can even be performed in complex mixtures such as cell extracts.