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Specific Fluorescent Probe for 8‐Oxoguanosine
Author(s) -
Nakagawa Osamu,
Ono Sayaka,
Li Zhichun,
Tsujimoto Akira,
Sasaki Shigeki
Publication year - 2007
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.200700671
Subject(s) - fluorescence , titration , clamp , chemistry , aqueous medium , solubilization , biophysics , computer science , aqueous solution , analytical chemistry (journal) , combinatorial chemistry , chromatography , biochemistry , physics , biology , optics , computer graphics (images) , clamping
Marking mutations : Complete discrimination of 8‐oxoguanosine (8‐oxoG) from other nucleosides has been achieved with a new fluorescent probe, named “8‐oxoG‐clamp” (see picture). The complex structure between 8‐oxoG‐clamp and 8‐oxoG was confirmed by 1 H NMR titration and 2D NMR measurements. A preliminary investigation with use of a detergent for solubilization indicates that 8‐oxoG‐clamp may be applicable in aqueous media.