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Quantum Dot/Bioluminescence Resonance Energy Transfer Based Highly Sensitive Detection of Proteases
Author(s) -
Yao Hequan,
Zhang Yan,
Xiao Fei,
Xia Zuyong,
Rao Jianghong
Publication year - 2007
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.200700280
Subject(s) - bioluminescence , quantum dot , energy transfer , förster resonance energy transfer , nanosensor , resonance (particle physics) , luciferase , nanotechnology , chemistry , physics , computer science , materials science , biochemistry , fluorescence , particle physics , engineering physics , quantum mechanics , gene , transfection
Sensing by BRET : Quantum dot (QD) nanosensors can detect the activity of matrix metalloproteinases by measuring the bioluminescence resonance energy transfer (BRET) efficiency between the QDs and a bioluminescent fusion protein (see scheme; Luc8= Renilla luciferase; His×6=six‐histidine tag).