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A Protein‐Responsive Chromophore Based on Squaraine and Its Application to Visual Protein Detection on a Gel for SDS‐PAGE
Author(s) -
Suzuki Yoshio,
Yokoyama Kenji
Publication year - 2007
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.200700245
Subject(s) - coomassie brilliant blue , chromophore , bovine serum albumin , detection limit , chemistry , chromatography , visible spectrum , blue light , photochemistry , staining , materials science , biology , optoelectronics , genetics
Dying to know : A visual protein indicator based on squaraine is responsible for a profound color change upon binding to protein in solution, from light pink for the dye alone to deep purple for the dye⋅BSA complex (see picture; BSA: bovine serum albumin). The detection limit for BSA is 2 μg mL −1 , which is about five times higher than that with Coomassie Brilliant Blue as the dye.