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Hydrogen Peroxide Dependent Monooxygenations by Tricking the Substrate Recognition of Cytochrome P450 BSβ
Author(s) -
Shoji Osami,
Fujishiro Takashi,
Nakajima Hiroshi,
Kim Misa,
Nagano Shingo,
Shiro Yoshitsugu,
Watanabe Yoshihito
Publication year - 2007
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.200700068
Subject(s) - hydrogen peroxide , substrate (aquarium) , chemistry , decoy , combinatorial chemistry , molecule , catalysis , monooxygenase , computer science , substrate specificity , cytochrome p450 , information retrieval , biochemistry , enzyme , organic chemistry , biology , ecology , receptor
Trick or treat : Cytochrome P450 BSβ was transformed into a monooxygenase suitable for practical use by employing a simple substrate trick. The substrate specificity of P450 BSβ was altered drastically by a decoy molecule, while its intrinsic advantage, the use of hydrogen peroxide, was retained. The catalytic activities and the enantioselectivity of the H 2 O 2 –P450 BSβ system are highly dependent on the structure of the decoy molecule.