Premium
Design of Molecular Logic Devices Based on a Programmable DNA‐Regulated Semisynthetic Enzyme
Author(s) -
Gianneschi Nathan C.,
Ghadiri M. Reza
Publication year - 2007
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.200700047
Subject(s) - library science , chemical biology , chemistry , computer science , biochemistry
Informed decisions : Logic operations (AND, OR, NOR) can be performed with an enzyme by tagging it and its inhibitor with single‐strand DNA. By adding appropriate single‐strand DNA, the activity of the enzyme complex can be switched ON and OFF (see picture for NOR logic; DE : DNA‐tagged enzyme; DI 1 , DI 2 : DNA‐tagged inhibitors), or it can be used as a sensitive PCR‐independent gene‐diagnostic probe.