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A Gold‐Nanoparticle‐Based Real‐Time Colorimetric Screening Method for Endonuclease Activity and Inhibition
Author(s) -
Xu Xiaoyang,
Han Min Su,
Mirkin Chad A.
Publication year - 2007
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.200605249
Subject(s) - endonuclease , colloidal gold , nanoparticle , cleavage (geology) , dna , chemistry , enzyme , biophysics , deoxyribonuclease , deoxyribonuclease i , deoxyribonucleases , nanotechnology , microbiology and biotechnology , biochemistry , materials science , biology , base sequence , fracture (geology) , composite material
On the small screen : Aggregates of gold nanoparticles interconnected by DNA duplexes (see scheme, left) are bluish‐purple. Cleavage of the duplexes by deoxyribonuclease I (DNase I) releases the nanoparticles (right), producing a bluish‐purple‐to‐red color change. This color change can be used to monitor the activity (and inhibition) of the enzyme.