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Surface‐Plasmon‐Enhanced Fluorescence Spectroscopy for DNA Detection Using Fluorescently Labeled PNA as “DNA Indicator”
Author(s) -
Chu LiQiang,
Förch Renate,
Knoll Wolfgang
Publication year - 2007
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.200605247
Subject(s) - dna , fluorescence , nucleic acid , fluorescence spectroscopy , peptide nucleic acid , spectroscopy , surface plasmon , chemistry , plasmon , homogeneous , nanotechnology , surface plasmon resonance , analytical chemistry (journal) , combinatorial chemistry , biophysics , biological system , materials science , chromatography , biochemistry , optoelectronics , biology , physics , optics , nanoparticle , quantum mechanics , thermodynamics
A homogeneous sensor : A new method for the detection of DNA is described. The approach is based on the high surface sensitivity provided by surface‐plasmon‐enhanced fluorescence spectroscopy and on the different electrostatic properties of peptide nucleic acids (PNAs) and DNA (see scheme). Immobilization of the DNA probes on the sensor surface is not required, which may reduce the complexity and costs of sensor preparation.