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DNA‐Based Carbohydrate Biochips: A Platform for Surface Glyco‐Engineering
Author(s) -
Chevolot Yann,
Bouillon Camille,
Vidal Sébastien,
Morvan François,
Meyer Albert,
Cloarec JeanPierre,
Jochum Anne,
Praly JeanPierre,
Vasseur JeanJacques,
Souteyrand Eliane
Publication year - 2007
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.200604955
Subject(s) - biochip , oligonucleotide , conjugate , carbohydrate , dna , lectin , chemistry , biochemistry , fluorescence , nanotechnology , combinatorial chemistry , computational biology , microbiology and biotechnology , computer science , materials science , biology , mathematics , physics , mathematical analysis , quantum mechanics
When the chips are down : A glycoarray consisting of oligonucleotide/carbohydrate conjugates hybridized onto a DNA chip can be used for the immobilization of oligosaccharides at extremely low concentrations (1 μ M ). With lectin, the lower detection limit of the device was 2–20 n m. The relative surface density of the conjugates was monitored by Cy3 fluorescence.