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Fluorescent Epibatidine Agonists for Neuronal and Muscle‐Type Nicotinic Acetylcholine Receptors
Author(s) -
Grandl Jörg,
Sakr Elias,
KotzybaHibert Florence,
Krieger Florian,
Bertrand Sonia,
Bertrand Daniel,
Vogel Horst,
Goeldner Maurice,
Hovius Ruud
Publication year - 2007
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.200604807
Subject(s) - epibatidine , nicotinic agonist , acetylcholine receptor , gating , acetylcholine , chemistry , biophysics , receptor , fluorescence , nicotinic acetylcholine receptor , neuroscience , ion channel , microbiology and biotechnology , biology , pharmacology , biochemistry , physics , quantum mechanics
Singled out : Specific high‐affinity binding to and activation of nicotinic acetylcholine receptors (nAChRs) by fluorescent epibatidine (EPB) agonists is detected by fluorescence microscopy and electrophysiology (see picture). The optical and pharmacological properties of these compounds allow single‐channel/single‐molecule experiments at the surface of living cells, and investigation of channel gating and receptor trafficking.