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Efficient Fluorescence Enhancement and Cooperative Binding of an Organic Dye in a Supra‐biomolecular Host–Protein Assembly
Author(s) -
Bhasikuttan Achikanath C.,
Mohanty Jyotirmayee,
Nau Werner M.,
Pal Haridas
Publication year - 2007
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.200604757
Subject(s) - fluorescence , bovine serum albumin , supramolecular chemistry , chemistry , biophysics , photochemistry , biochemistry , organic chemistry , biology , molecule , physics , quantum mechanics
A factor of 300 enhancement in fluorescence and increased binding is observed when the triphenylmethane dye Brilliant Green binds to bovine serum albumin (BSA) in the presence of the macrocyclic host cucurbit[7]uril (CB7) as a supramolecular “enhancer” (see picture). Thus, a cumulative (multiplicative) fluorescence enhancement occurs compared to the effects observed in the presence of CB7 alone (factor of 6) or BSA alone (factor of 45).