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O‐Glycoside Orientation Is an Essential Aspect of Base J Recognition by the Kinetoplastid DNA‐Binding Protein JBP1
Author(s) -
Grover Rajesh K.,
Pond Stephanie J. K.,
Cui Qizhi,
Subramaniam Prem,
Case David A.,
Millar David P.,
Wentworth Paul
Publication year - 2007
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.200604635
Subject(s) - dna , trypanosoma , dissociation constant , stereochemistry , chemistry , biology , biochemistry , genetics , receptor
A binding edge? Comparison of dissociation constants and molecular‐dynamics snapshots of a panel of synthetic telomeric double‐stranded DNA sequences containing HMdU O‐glycosides has revealed that the DNA‐binding protein of kinetoplastids, such as Leishmania and Trypanosoma , JBP1, recognizes a critical edge‐on conformation of the pyranosyl ring of base J. If this conformation is perturbed, JBP1 binding is dramatically reduced.