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Two‐Photon Uncaging with the Efficient 3,5‐Dibromo‐2,4‐dihydroxycinnamic Caging Group
Author(s) -
Gagey Nathalie,
Neveu Pierre,
Jullien Ludovic
Publication year - 2007
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.200604598
Subject(s) - substrate (aquarium) , fluorescence , two photon excitation microscopy , chemistry , photochemistry , ethanol , excitation , in vivo , optoelectronics , materials science , optics , physics , biology , biochemistry , ecology , microbiology and biotechnology , quantum mechanics
An optical syringe for targeted delivery of a substrate in vivo has been developed on the basis of the 3,5‐dibromo‐2,4‐dihydroxycinnamic caging group. Two‐photon excitation of the caged compound uncages the substrate (ethanol in the scheme) and forms a coumarin whose fluorescence allows the concentration of released substrate to be quantified. Single‐cell release can be achieved with a focused laser (shown schematically for a zebrafish embryo).