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Detecting Force‐Induced Molecular Transitions with Fluorescence Resonant Energy Transfer
Author(s) -
Tarsa Peter B.,
Brau Ricardo R.,
Barch Mariya,
Ferrer Jorge M.,
Freyzon Yelena,
Matsudaira Paul,
Lang Matthew J.
Publication year - 2007
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.200604546
Subject(s) - förster resonance energy transfer , optical tweezers , fluorescence , resonant inductive coupling , energy transfer , fluorescence microscope , microscopy , tweezers , fluorescence in the life sciences , biophysics , chemistry , materials science , nanotechnology , optics , molecular physics , physics , biology
Don't FRET : The first successful combination of optical‐tweezers force microscopy and single‐molecule fluorescence resonant energy transfer (FRET) is demonstrated with a force sensor based on a DNA hairpin (see picture). As the hairpin is opened and closed by the optical tweezers, the structural change is simultaneously monitored by the FRET emission from fluorescence labels.