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The Chemical Composition of Animal Cells and Their Intracellular Compartments Reconstructed from 3D Mass Spectrometry
Author(s) -
Breitenstein Daniel,
Rommel Christina E.,
Möllers Rudolf,
Wegener Joachim,
Hagenhoff Birgit
Publication year - 2007
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.200604468
Subject(s) - mass spectrometry , secondary ion mass spectrometry , substrate (aquarium) , sputtering , chemistry , layer (electronics) , analytical chemistry (journal) , chemical composition , ion , molecule , peek , mass spectrometry imaging , biophysics , materials science , chromatography , nanotechnology , biology , organic chemistry , thin film , ecology , polymer
A peek inside : A C 60 + sputter ion source integrated into a time‐of‐flight secondary‐ion mass spectrometer was used to dissect animal cells in a layer‐by‐layer fashion and analyze the spatial distribution of endogenous molecules. Repeated cycles of sputter erosion and SIMS analysis provided molecular information from the inside of the cells (see picture; red: amino acids, green: phospholipids, blue: glass substrate).
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