Premium
A Nucleoside That Contains a Rigid Nitroxide Spin Label: A Fluorophore in Disguise
Author(s) -
Barhate Nivrutti,
Cekan Pavol,
Massey Archna P.,
Sigurdsson Snorri Th.
Publication year - 2007
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.200603993
Subject(s) - nitroxide mediated radical polymerization , nucleoside , fluorophore , spin label , chemistry , electron paramagnetic resonance , nucleic acid , site directed spin labeling , spin (aerodynamics) , fluorescence , combinatorial chemistry , stereochemistry , nuclear magnetic resonance , organic chemistry , physics , biochemistry , quantum mechanics , radical polymerization , thermodynamics , copolymer , polymer
Take a spin : The nucleoside Ç (C‐spin), which contains a rigid nitroxide spin label, allows the structure and dynamics of nucleic acids into which it is incorporated to be studied by EPR spectroscopy (left spectrum). Reduction of the nitroxide with a mild reducing agent renders the nucleoside fluorescent (right spectrum). Thus, the same nucleic acid can be studied with two complementary spectroscopic techniques.